Induced regeneration in mouse digits

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Can limb regeneration be induced? Few have pursued this question, and an evolutionarily conserved strategy has yet to emerge. This study reports a strategy for inducing regenerative response in appendages, which works across three species that span the animal phylogeny. In Cnidaria, the frequency of appendage regeneration in the moon jellyfish Aurelia was increased by feeding with the amino acid L-leucine and the growth hormone insulin. In insects, the same strategy induced tibia regeneration in adult Drosophila. Finally, in mammals, L-leucine and sucrose administration induced digit regeneration in adult mice, including dramatically from mid-phalangeal amputation. The conserved effect of L-leucine and insulin/sugar suggests a key role for energetic parameters in regeneration induction. The simplicity by which nutrient supplementation can induce appendage regeneration provides a testable hypothesis across animals.

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This deposit contains the images representing phenotypes observed in induced mouse digit regeneration in the manuscript, 'A conserved strategy for inducing appendage regeneration.' This zip archive contains all Alizarin Red/Alcian blue stained mouse digit images (both the digit and the phalanges amputated), representing all the digit phenotypes as quantified in the tables presented in Figure 6—figure supplement 1. All digits were imaged in 100% glycerol, on a Zeiss Stemi 305 dissection microscope with an iPhone 6 camera. Please see the related publication for more details, and contact the corresponding authors for any questions.

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Related Publication: A conserved strategy for inducing appendage regeneration Michael J Abrams UC Berkeley Fayth Hui Tan Caltech Ty Basinger Bloomsburg University Martin L Heithe Caltech Yutian Li Caltech Misha Raffiee Stanford University Patrick Leahy Caltech John O Dabiri Caltech David A Gold UC Davis Lea A Goentoro Caltech https://doi.org/10.1101/2020.11.21.392720 eng

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