Published 2018 | Version v1
Dataset Open

gh2-4_sq2_cell5

  • 1. ROR icon California Institute of Technology

Description

GH2-4, Sq2, IT 1s, phase plate

Technical info

Tilt Series Date: 2018-05-17

Data Taken By: William Nicolas

Species / Specimen: Gluconacetobacter hansenii

Strain: ATCC 23769

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 48.0°), step: 2°, constant angular increment, dosage: 164.0 eV/Ų, defocus: -2.0 μm, magnification: 33000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h

Sample Preparation: Backside manual blotting 5s

Attached Files

gh2-4_sq2_cell5.mrc, Tilt Series (Pixel Size 0.43 nm), 1.6 GB

gh2-4_sq2_cell5_part55_2.rec, Reconstruction (Pixel Size 0.86 nm), 2.1 GB

Files

keyimg_wnj2018-05-17-1.jpg

Files (3.7 GB)

Name Size
1.6 GB (external) Download
2.1 GB (external) Download
md5:9c46d2e6e5249ee11a4363953fb62824
4.3 MB Preview Download
md5:d6b7019eeaa744693846c39ea984ba5d
5.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/qzcrp-te432
TILT_SERIES_ID
wnj2018-05-17-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2018-05-17
Created
2018-05-19
Updated
2018-05-25