Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

T5_504Cys_Aumalemaide_5nm

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

This is a sample in which N terminus residue 504 has been mutated to Cys residue. Mini cells have been isolated by high speed centrifugation and cells are Au malemaide labelled. No fiducials added.

Technical info

Tilt Series Date: 2017-02-17

Data Taken By: Poorna Subramanian

Species / Specimen: Escherichia coli

Strain: EC93 stick, UPEC536

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: autoPatch

Collaborators and Roles: Zach Ruhe and Chris Hayes

Purification / Growth Conditions / Treatment: Freezing in vitrobot. Humidifier was not working. I wouldn't really trust the conditions.See pages 104-105

Sample Preparation: Sample prepared by Taylor Steven

Attached Files

sq120008.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB

sq120008.rec, Reconstruction (Pixel Size 1.56 nm), 1.1 GB

Files

keyimg_psa2017-02-17-38.jpg

Files (4.8 GB)

Name Size
md5:af98a5c8311c911186e80023c158a566
998.9 kB Preview Download
md5:09835e69fa0de70a23ae70096f4d9431
1.8 MB Preview Download
3.7 GB (external) Download
1.1 GB (external) Download

Additional details

Identifiers

DOI
10.22002/r3343-5ze74
TILT_SERIES_ID
psa2017-02-17-38

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-02-17
Created
2017-02-18
Updated
2019-03-25