Published 2016
| Version v1
Dataset
Open
JV5443
Description
JV5443 Legionella (Super-deletion+CDHFG), no Thy
Files
keyimg_dga2016-08-09-24.jpg
Technical info
Tilt Series Date: 2016-08-09
Data Taken By: Debnath Ghosal Species / Specimen: Legionella pneumophila Strain: Philadelphia-1_Lp02 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x. Microscope: Janelia Titan Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal at Janelia Krios. Purification / Growth Conditions / Treatment: Cells were grown in AYE liquid culture with following supplements: ferric nitrate and cysteine hydrochloride (no thymidine). Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0. Sample Preparation: 8 grids were frozen for the JV5443 strain at OD~3.2. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-08-09-24/rawdata/
JV5443a0077.mrc, Tilt Series (Pixel Size 0.42 nm), 2.3 GB Download JV5443a0077_full.rec, Reconstruction (Pixel Size 1.68 nm), 445.4 MB DownloadAdditional details
- DOI
- 10.22002/raqs5-hy238
- TILT_SERIES_ID
- dga2016-08-09-24
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2016-08-09
- Created
-
2016-08-10
- Updated
-
2016-08-15