Published 2013 | Version v1
Dataset Open

Tsr oe vesicles:W:A 1.5:0.5:1

  • 1. ROR icon California Institute of Technology

Contributors

Description

Tsr overexpression vesicles with 1.5:0.5:1 Tst:W:A incubation for ternary complex formation

Technical info

Tilt Series Date: 2013-03-14

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 19500x.

Acquisition Software: FEIbatchtomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Laura Kiessling, Heather Hodges

Purification / Growth Conditions / Treatment: Buffer Tsr reaction buffer (50 mM Tris-HCl pH 7.4 50mM KCl 5mM MgCl2 0.5 Mm TCEP

Attached Files

HLH3_031313_3_part67_3.rec, Reconstruction (Pixel Size 1.22 nm), 838.9 MB

HLH3_031313_3.mrc, Tilt Series (Pixel Size 0.61 nm), 562.0 MB

Files

keyimg_aba2013-03-14-10.jpg

Files (1.4 GB)

Name Size
562.0 MB (external) Download
838.9 MB (external) Download
md5:0e3e2fe5ece7b655ab4c0ec2eaeff5b5
1.3 MB Preview Download
md5:9e1deffa066186de0c8c08479ce583bf
31.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/rat54-w6z21
TILT_SERIES_ID
aba2013-03-14-10

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-03-14
Created
2013-03-20
Updated
2013-03-22