Published 2012 | Version v1
Dataset Open

UU 2804- A413T CheA deltaP2

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

E. coli strain A413T (locked in CheA off state) with deletion of P2 in CheA

Technical info

Tilt Series Date: 2012-08-31

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain: RP 437

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 34000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFtomo/IMOD/tomo3D

Collaborators and Roles: John S. Parkinson

Purification / Growth Conditions / Treatment: cells grown to log phase in TB media, lysed with penicillin for one hour

Sample Preparation: plunge frozen on R2/2 Quantifoil grids in liquid ethane/propane

Attached Files

2804atlas10005.mrc, Tilt Series (Pixel Size 0.33 nm), 3.7 GB

2804atlas10005_full.rec, Reconstruction (Pixel Size 0.66 nm), 2.8 GB

Files

keyimg_aba2012-08-31-5.jpg

Files (6.5 GB)

Name Size
3.7 GB (external) Download
2.8 GB (external) Download
md5:0ea2d4a32133f9260455abfede8505a5
4.3 MB Preview Download
md5:db693e80134e5fbc6e757c3a492424cc
13.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/rayp3-b5979
TILT_SERIES_ID
aba2012-08-31-5

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-08-31
Created
2012-08-31
Updated
2012-11-19