Published 2015 | Version v1
Dataset Open

E. coli Sacculi +A22 (MG1655)

Creators

  • 1. ROR icon California Institute of Technology

Description

Raw data files of E. coli Sacculi +A22 (MG1655)

Technical info

Tilt Series Date: 2015-04-10

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain: MG1655 sacculi

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: raptor

Sample Preparation: Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.

Attached Files

041015_mg1655_a22_atlas4_tomo0002.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

041015_mg1655_a22_atlas4_tomo0002_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB

Files

keyimg_mst2015-04-10-39.jpg

Files (3.9 GB)

Name Size
3.4 GB (external) Download
445.4 MB (external) Download
md5:b9929c4470c3360eeb9d26ccb3c51ded
1.0 MB Preview Download
md5:00429e5a7c2d2dbc93f778466d095d7a
15.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/rcd3s-4rz68
TILT_SERIES_ID
mst2015-04-10-39

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-04-10
Created
2015-04-13
Updated
2022-11-03