V.cholerae
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2017-01-06
Data Taken By: Poorna Subramanian
Species / Specimen: Vibrio cholerae
Strain: O1 biovar El Tor
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Yi-wei
Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.
psa2017-01-06-42_slicer17804.jpg: 2 basal bodies-one with and one w/o flagellum psa2017-01-06-42_slicer17805.jpg: DNA, cell is lysed psa2017-01-06-42_slicer17806.jpg: DNA, cell is lysed psa2017-01-06-42_slicer17807.jpg: DNA, cell is lysed psa2017-01-06-42_slicer17808.jpg: DNA, cell is lysed
Attached Files
atlas50020_part121_1.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
atlas50020.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
Files
keyimg_psa2017-01-06-42.jpg
Files
(4.2 GB)
| Name | Size | |
|---|---|---|
| 3.4 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:b8065b0b7c63232175321d680572bfc4
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1.1 MB | Preview Download |
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md5:d32fce21681809e50fa01979ff9f311a
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23.5 MB | Preview Download |
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md5:f7e931aa9e23846a4b95f16e29a1972c
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329.0 kB | Preview Download |
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md5:328b1e8b6950eaae26fa88686e735f4d
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340.7 kB | Preview Download |
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md5:64d52987fd2420d2d8a12228a4ea7385
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363.5 kB | Preview Download |
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md5:061855b73571d0d8fd3f589d59087139
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354.6 kB | Preview Download |
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md5:1d7b91838b836a00772648fd9b498d23
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354.9 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/rf0fd-6ze18
- TILT_SERIES_ID
- psa2017-01-06-42
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-01-06
- Created
-
2017-01-08
- Updated
-
2017-04-05