Halobacterium salinarum

Description

Technical info

<strong>Tilt Series Date:</strong> 2016-12-16</p>

<strong>Data Taken By:</strong> Debnath Ghosal</p>

<strong>Species / Specimen:</strong> Halobacterium salinarum</p>

<strong>Strain:</strong> </p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x. </p>

<strong>Microscope:</strong> Caltech Polara</p>

<strong>Acquisition Software:</strong> UCSFTomo</p>

<strong>Upload Method:</strong> pipeline</p>

<strong>Processing Software Used:</strong> RAPTOR</p>

<strong>Collaborators and Roles:</strong> Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal</p>

<strong>Purification / Growth Conditions / Treatment:</strong> Standard H. salinarum medium</p>

<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.</p>

dga2016-12-16-9_slicer17295.jpg: spindle-shaped gas vesicles

External Files

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-12-16-9/3dimage_80642/Hs_201612160008_full.rec" > <i class="download icon"></i> Download </a>

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-12-16-9/rawdata/Hs_201612160008.mrc" > <i class="download icon"></i> Download </a>