Published 2015 | Version v1
Dataset Open

nanochemowt

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.

Technical info

Tilt Series Date: 2015-06-02

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Batchruntomo

Collaborators and Roles: Moh and Sahand USC

Sample Preparation: This sample was frozen after being in oxygen deficiency for 16h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.

Attached Files

nanochemo_atlas10019.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

nanochemo_atlas10019.rec, Reconstruction (Pixel Size 1.92 nm), 1.1 GB

Files

keyimg_psa2015-06-02-8.jpg

Files (4.5 GB)

Name Size
md5:c458f77657511dbaf86555afff3573f9
841.2 kB Preview Download
md5:4918e0c68580b692fb2c7d7eef566bb0
18.9 MB Preview Download
3.4 GB (external) Download
1.1 GB (external) Download

Additional details

Identifiers

DOI
10.22002/rqhaj-jst30
TILT_SERIES_ID
psa2015-06-02-8

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-06-02
Created
2015-06-02
Updated
2015-06-07