Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

wt_perfusion_fixed_immunofluoro

Creators

  • 1. ROR icon California Institute of Technology

Description

This sample is wt cells grown in perfusion set-up. It was imaged on LM for wires, fixed with 4 % formaldehyde for 1 hour, immunofluorescence was done for cytochromes, reimaged, fixed with 2.5% glutaraldehyde for 15 minutes, triple washed with distilled water and saved in distilled water until freezing. The grid was prepared by Sahand on 10/22/15. The grid was frozen on 10/23/15 at Caltech using vitrobot MK4.

Technical info

Tilt Series Date: 2015-10-27

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -5.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: motion correction was done during collection @ 5 frames/tilt angle

Collaborators and Roles: Moh and Sahand USC

Sample Preparation: The sample was frozen using vitrobot. C side left. 1.8 ul gold 10nm size was added on opposite side while on vitrobot. conditions: blot force=6, blot time=2.5s, drain time =1s, wait time=0. humidity set 80%. temp 22C. Use 2.5ul gold next time

Attached Files

manual4_sq10-1_mcorr_full.rec, Reconstruction (Pixel Size 1.28 nm), 445.4 MB

manual4_sq10-1_mcorr.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

Files

keyimg_psa2015-10-27-23.jpg

Files (3.9 GB)

Name Size
md5:ffdd072e1332a2a8eaa6fb799dbd91bf
1.1 MB Preview Download
md5:78a7a86e82049506580708b83a2225fe
15.2 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/rv48b-n0a12
TILT_SERIES_ID
psa2015-10-27-23

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-10-27
Created
2015-11-18
Updated
2016-01-04