Legionella_JV9246
Description
Technical info
Tilt Series Date: 2019-05-28
Data Taken By: Debnath Ghosal
Species / Specimen: Legionella pneumophila
Strain: Philadelphia-1_Lp02
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -4.0 μm, magnification: 42000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected by Debnath Ghosal and Yuxi Liu.
Purification / Growth Conditions / Treatment: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.
Attached Files
JV9246_area1_tomo0025.mrc, Tilt Series (Pixel Size 0.26 nm), 1.2 GB
JV9246_area1_tomo0025_part41_6.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB
Files
keyimg_dga2019-05-28-50.jpg
Additional details
Identifiers
- DOI
- 10.22002/rv4ak-fxv97
- TILT_SERIES_ID
- dga2019-05-28-50
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-05-28
- Created
-
2019-06-03
- Updated
-
2019-06-03