Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

COS7 CHMP4B+VPS4DN

Woodward, Cora1
  • 1. ROR icon California Institute of Technology
Researchers:
Sundquist, Wes
Woodward, Cora

Description

Cos7 cells transfected with CHMP4B & VPS4DN constructs

Files

keyimg_cwl2015-03-08-19.jpg
Files (23.5 MB)
Name Size
keymov_cwl2015-03-08-19.mp4
md5:7413bb8541d17e9c24028428aacffae5
22.4 MB Download
keyimg_cwl2015-03-08-19.jpg
md5:9abb653a9e607faaed2f97451a6d420f
1.1 MB Preview Download

Technical info

Tilt Series Date: 2015-03-08

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with Lipofectamine

Sample Preparation: blotting

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2015-03-08-19/rawdata/

15mar08_C301-1_AT3BT1_0011.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB Download

15mar08_C301-1_AT3BT1_0011_full.rec, Reconstruction (Pixel Size 2.0 nm), 623.6 MB Download

Additional details

Identifiers Funding Dates
Created:
May 26, 2023
Modified:
August 28, 2025