Published 2013 | Version v1
Dataset Open

HUVECs infx pNLGFP

  • 1. ROR icon California Institute of Technology

Description

single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 150/A2. defocus: -6um. magnification: 19000.

Technical info

<strong>Tilt Series Date:</strong> 2013-07-05</p>

<strong>Data Taken By:</strong> Cora Woodward</p>

<strong>Species / Specimen:</strong> HUVECs</p>

<strong>Strain:</strong> </p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 19000x. </p>

<strong>Microscope:</strong> Janelia Titan</p>

<strong>Acquisition Software:</strong> UCSFTomo</p>

<strong>Upload Method:</strong> pipeline</p>

<strong>Processing Software Used:</strong> UCSFTomo</p>

<strong>Collaborators and Roles:</strong> pNLGFP obtained from Irvin Chen (UCLA)</p>

<strong>Sample Preparation:</strong> Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection</p>

External Files

Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-14/rawdata/</p>

13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0015.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-14/rawdata/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0015.mrc" > <i class="download icon"></i> Download </a>

</p>

13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0015_full.rec, Reconstruction (Pixel Size 2.24 nm), 602.8 MB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2013-07-05-14/3dimage_28040/13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0015_full.rec" > <i class="download icon"></i> Download </a>

</p>

Files

keyimg_cwl2013-07-05-14.jpg

Files (23.1 MB)

Name Size
md5:4039dc5432404d0e9bd1bf4a9b9d06ad
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md5:82f7529b155da39fc62bf6473ce2aea9
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Additional details

Identifiers

DOI
10.22002/rz2dk-5b445
TILT_SERIES_ID
cwl2013-07-05-14

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-07-05
Created
2013-07-06
Updated
2019-03-06