Myxococcus xanthus delta-pilB

Description

Technical info

Tilt Series Date: 2013-12-31

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.

ycw2013-12-31-36_slicer9018.jpg: ring . Myxococcus xanthus ycw2013-12-31-36_slicer9021.jpg: Type VI Secretion System? . Myxococcus xanthus ycw2013-12-31-36_slicer10547.jpg: Myxococcus xanthus T6SS contracted side view and top view

Attached Files

20131231_YWC066_10036.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB

20131231_YWC066_10036_part131_8.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB