Published 2016 | Version v1
Dataset Open

NR1769/R1-16 conjugation

Creators

  • 1. ROR icon California Institute of Technology

Description

Grid box YWC228NR1769/R1-16 conjugating with NR1769 skinny E. coli

Technical info

Tilt Series Date: 2016-11-01

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain and sample prepared by Carrie Shaffer

ycw2016-11-01-16_slicer17102.jpg: R1-16 tube? ycw2016-11-01-16_slicer17103.jpg: R1-16 machines ycw2016-11-01-16_slicer17148.jpg: assembled R1-16 spanning 2 membranes ycw2016-11-01-16_slicer17149.jpg: adjacent membrane tubes

Attached Files

20161101_YWC228_10016.mrc, Tilt Series (Pixel Size 0.48 nm), 2.3 GB

20161101_YWC228_10016_full.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_ycw2016-11-01-16.jpg

Files (2.9 GB)

Name Size
2.3 GB (external) Download
534.5 MB (external) Download
md5:3bee680d1b8e0bda68592a6f91d1f2b9
1.1 MB Preview Download
md5:fee870fc4ac64da5c1c9cb8a8f35b797
11.6 MB Preview Download
md5:765d80ecc085f09c488a31a52c56ef42
309.2 kB Preview Download
md5:7ac2f94bf47d288e5f8dfbc6549b93c3
268.5 kB Preview Download
md5:0f0a9ab4f25ae7039b8c8a38e2ca57db
235.5 kB Preview Download
md5:8d8ab48f6d46701f2726ba3d89f1bbd0
278.3 kB Preview Download

Additional details

Identifiers

DOI
10.22002/s8kvc-wn936
TILT_SERIES_ID
ycw2016-11-01-16

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-11-01
Created
2016-11-01
Updated
2016-11-15