Published 2016 | Version v1
Dataset Open

Sec61-GFP CLEM INS-1E Cells

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

INS-1E cells grown on R2/2 London finder grids and transfected with Sec61-GFP

Technical info

Tilt Series Date: 2016-09-17

Data Taken By: Stephen Carter

Species / Specimen: INS-1E Pancreatic Cells expressing Sec61-GFP

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -9.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: IMOD and SIRT

Collaborators and Roles: Zachary Freyberg

Attached Files

tomography7.rec, Reconstruction (Pixel Size 24.0 nm), 2.7 GB

tomography7.mrc, Tilt Series (Pixel Size 6.0 nm), 3.4 GB

Files

keyimg_scd2016-09-17-8.jpg

Files (6.2 GB)

Name Size
md5:beee909628ddfafdc37a69398eee2c26
1.0 MB Preview Download
md5:64c52e42a31ed484d0f0826509cb3983
33.0 MB Preview Download
3.4 GB (external) Download
2.7 GB (external) Download

Additional details

Identifiers

DOI
10.22002/s9qtf-hjc68
TILT_SERIES_ID
scd2016-09-17-8

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-09-17
Created
2016-09-20
Updated
2017-12-20