Published 2015
| Version v1
Dataset
Open
Pseudomonas aer2 in E.coli
Contributors
Researcher:
Description
Aer2 expressed from pProEx plasmid = amp resistant, in E. coli BT3388
Technical info
Tilt Series Date: 2015-03-13
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 8.0 μm, magnification: 34000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Kylie Watts, Loma Linda University
Purification / Growth Conditions / Treatment: grown with 0.5 ml of 0.1% thiamine per L of LBAmp
Attached Files
Aer2inEcoliTomo0003.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB
Aer2inEcoliTomo0003_part121_60.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB
Files
keyimg_aba2015-03-13-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/sa246-0wj74
- TILT_SERIES_ID
- aba2015-03-13-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-03-13
- Created
-
2015-03-13
- Updated
-
2015-09-21