Azo delta Operon 1
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-02-12
Data Taken By: Ariane Briegel
Species / Specimen: Azospirillum brasilense
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 10.0 μm, magnification: 34000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Batchruntomo
Collaborators and Roles: Gladys Alexandre (University of Tennessee)
Purification / Growth Conditions / Treatment: Grow overnight in 5 mL MMAB+N without shakingSpin down 10' at 3,500 x gResuspend pellet in 5 mL MMAB-NSpin down 10' at 3,500 x gResuspend pellet in 5 mL MMAB-N + 200 μg/mL AmpicillinLeave at RT (on bench) without shaking overnight
Attached Files
AzoDeltaOp1Feb0012.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB
AzoDeltaOp1Feb0012.rec, Reconstruction (Pixel Size 1.28 nm), 1.4 GB
Files
keyimg_aba2015-02-12-40.jpg
Additional details
Identifiers
- DOI
- 10.22002/scth8-rqp63
- TILT_SERIES_ID
- aba2015-02-12-40
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-02-12
- Created
-
2015-02-13
- Updated
-
2015-02-25