Oil well Actinobacterium

Description

Technical info

Tilt Series Date: 2016-04-11

Data Taken By: Yi-Wei Chang

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 75.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Fixed cells received from Meng Wang/ Xiao-Lei Wu Lab at Peking University, China

Purification / Growth Conditions / Treatment: Firstly wash cells using PBS for 3 times, then add proper volum of 2.5% glutaraldehyde followed 1 hour room temp incubation (done in Peking), then send to Caltech for freezing.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

Attached Files

20160410_YWC210_10013.mrc, Tilt Series (Pixel Size 0.48 nm), 996.7 MB

20160410_YWC210_10013_part35_1.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB