Published 2009 | Version v1
Dataset Open

Hyphomonas session20 34KX

Creators

  • 1. ROR icon California Institute of Technology

Description

high-order DNA, H-NS, nucleoid, asymmetric cell division

Technical info

Tilt Series Date: 2009-07-13

Data Taken By: Jian Shi

Species / Specimen: Hyphomonas neptunium

Strain: ATCC 15444

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: raptor

Collaborators and Roles: Cultured by Jian; data collected by Jian; processed by Jian

Purification / Growth Conditions / Treatment: exponential phase in 2216 media, filtered flowthrough grow long time at 4degree...

Sample Preparation: QF grid, 10nm gold, 100% humidity...

jsa2009-07-13-4_slicer8800.jpg: DNA supercoil . Hyphomonas neptunium

Attached Files

09jul13a_b624a_004.mrc, Tilt Series (Pixel Size 0.632 nm), 553.7 MB

09jul13a_b624a_004_full.rec, Reconstruction (Pixel Size 1.264 nm), 367.0 MB

Files

jsa2009-07-13-4_slicer8800.jpg

Files (933.7 MB)

Name Size
553.7 MB (external) Download
367.0 MB (external) Download
md5:fd9e4c4c0df1701fbda480caf04bacfe
309.6 kB Preview Download
md5:bdbaf69a57f9ce5afe7c311ca93e4f62
1.1 MB Preview Download
md5:a5a6e664d2b048e5a3fe1450da994abe
11.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/shgye-fd239
TILT_SERIES_ID
jsa2009-07-13-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-07-13
Created
2009-10-20
Updated
2014-07-29