Hpylori_dFliF_FISE
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2020-10-08
Data Taken By: Mohammed Kaplan
Species / Specimen: Helicobacter pylori
Strain: 26695
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -8.0 μm, magnification: 19500x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: The cells were prepared and sent by Carrie Schaffer from the university of Kentucky.
Purification / Growth Conditions / Treatment: The cells were grown at 37C with 5% CO2 on sheep blood agar plates for 2 days from a -80C . stock. No growth was seen on the plates after 24 hr therefore the plates were kept in the . incubator for another 24Hr.
Sample Preparation: The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100% humidity. Cu grids. 20 nm . gold.
Attached Files
Hpylori_DFliF_FISE_159.mrc, Tilt Series (Pixel Size 0.452 nm), 1.9 GB
Hpylori_DFliF_FISE_159_full.rec, Reconstruction (Pixel Size 1.808 nm), 737.3 MB
Files
keyimg_mka2020-10-08-156.jpg
Additional details
Identifiers
- DOI
- 10.22002/sjcek-pzd04
- TILT_SERIES_ID
- mka2020-10-08-156
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2020-10-08
- Created
-
2020-10-13
- Updated
-
2022-09-28