H. pylori 26695 / Hs.746T

Description

Technical info

Tilt Series Date: 2013-12-16

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori 26695 from ATCC, prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4 Hs.746T cells cultivated on QF Au finder grid for 2 day in RPMI + 10% FBS. Infect by pylori for 3 hr.

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (0.8 ml gold resuspent in 30 ul RPMI+10% FBS). Manual blot by Whatman 40 filter paper. Ethane/propane mix.

ycw2013-12-16-11_slicer4001.jpg: H.pylori. Double layer of filaments at division plane. ycw2013-12-16-11_slicer4002.jpg: H.pylori. Double layer of filaments at division plane. Side view. ycw2013-12-16-11_slicer4255.jpg: Dividing ycw2013-12-16-11_slicer4256.jpg: Extracellular vesicle ycw2013-12-16-11_slicer4259.jpg: Division rings ycw2013-12-16-11_slicer4262.jpg: External vesicle ycw2013-12-16-11_slicer4266.jpg: Granule

Attached Files

20131216_YWC057_10006.mrc, Tilt Series (Pixel Size 0.48 nm), 2.5 GB

20131216_YWC057_10006_part87_2.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB