Published 2018
| Version v1
Dataset
Open
INS-1E PBC
Contributors
Researcher:
Description
Untransfected INS-1E cells
Technical info
Tilt Series Date: 2018-07-15
Data Taken By: Stephen Carter
Species / Specimen: Rat INS-1E pancreatic beta cells
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Microscope: Caltech Titan Krios
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Ray Stevens
Purification / Growth Conditions / Treatment: INS-1E cells grown on gold finder grids, stimulated with glucose.
Sample Preparation: Manual Blotting
Attached Files
tomography8.mrc, Tilt Series (Pixel Size 3.26 nm), 3.0 GB
tomography8_full.rec, Reconstruction (Pixel Size 0.0 nm), 668.2 MB
Files
keyimg_scd2018-07-15-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/srhac-jwh58
- TILT_SERIES_ID
- scd2018-07-15-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-07-15
- Created
-
2021-01-25
- Updated
-
2021-03-30