Vibrio cholerae MKW1383

Description

Technical info

Tilt Series Date: 2013-05-01

Data Taken By: Yi-Wei Chang

Species / Specimen: Vibrio cholerae

Strain: 2740-80

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Acquisition Software: UCSF Tomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor

Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.

ycw2013-05-01-18_slicer9830.jpg: Vibrio cholerae . chemoreceptor array

Attached Files

grid3-tiltseries0018.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB

grid3-tiltseries0018_full.rec, Reconstruction (Pixel Size 1.68 nm), 516.7 MB