Published 2014 | Version v1
Dataset Open

HeLa

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Looking for midbodies

Technical info

Tilt Series Date: 2014-01-05

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: with Doug Mckay

Sample Preparation: HeLa cells+siRNA NUP153 + Thymidine. Freeze after 14 hours of thymidine release.

Attached Files

30008.mrc, Tilt Series (Pixel Size 0.6 nm), 448.7 MB

30008.rec, Reconstruction (Pixel Size 2.4 nm), 313.1 MB

Files

keyimg_rra2014-01-05-24.jpg

Files (769.1 MB)

Name Size
448.7 MB (external) Download
313.1 MB (external) Download
md5:cf88538ee5aa1274d98053bd915ea326
271.2 kB Preview Download
md5:038fb9639f2005822803c3089c548676
7.0 MB Preview Download

Additional details

Identifiers

DOI
10.22002/st18e-w9a36
TILT_SERIES_ID
rra2014-01-05-24

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-01-05
Created
2015-01-06
Updated
2015-01-12