Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

Rhesus Macaque Fibroblast

Carter, Stephen1
  • 1. ROR icon California Institute of Technology

Description

Express native levels of untagged TRIM5α. Can see RAVs in fibroblasts for the first time. Most of these cells actually cells displayed mainly auto fluorescent puncta and probably did not correlate any virus because light image only taken in the red channel.

Files

keyimg_scd2015-04-20-3.jpg
Files (32.6 MB)
Name Size
keymov_scd2015-04-20-3.mp4
md5:63e2e008f16a873b5c190029530ea120
31.5 MB Download
keyimg_scd2015-04-20-3.jpg
md5:7780fade192a640d19b92986e972988f
1.1 MB Preview Download

Technical info

Tilt Series Date: 2015-04-20

Data Taken By: Stephen Carter

Species / Specimen: Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope

Purification / Growth Conditions / Treatment: Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift.

External Files

Files available via S3 at s://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/scd2015-04-20-3/rawdata/

tomography3.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB Download

tomography3_full.rec, Reconstruction (Pixel Size 2.4 nm), 1.1 GB Download

Additional details

Identifiers Funding Dates
Created:
May 29, 2023
Modified:
August 28, 2025