CenK Sacculus

Description

Technical info

Tilt Series Date: 2007-05-05

Data Taken By: Lu Gan

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-70.0°, 70.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan; collaboration with Jeff Skerker and Michael Laub at MIT

Purification / Growth Conditions / Treatment: Depleted 40 hours in PYE + 0.1% glucose; Purified by hot SDS

Sample Preparation: C-flat 2/2, BSA treated 10nm gold, 100% humidity, -4mm offset,

Attached Files

CenK_05.mrc, Tilt Series (Pixel Size 0.96 nm), 1.2 GB

CenK_05.rec, Reconstruction (Pixel Size 0.96 nm), 561.3 MB