Published 2009 | Version v1
Dataset Open

Hyphomonas session15 27.5KX

Creators

  • 1. ROR icon California Institute of Technology

Description

high-order DNA, H-NS, nucleoid, asymmetric cell division

Technical info

Tilt Series Date: 2009-06-05

Data Taken By: Jian Shi

Species / Specimen: Hyphomonas neptunium

Strain: ATCC 15444

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: raptor

Collaborators and Roles: Cultured by Jian; data collected by Jian; processed by Jian

Purification / Growth Conditions / Treatment: exponential phase in PYE media, chloroquine 0.1mg/ml...

Sample Preparation: QF grid, 10nm gold, 100% humidity...

jsa2009-06-05-37_slicer8975.jpg: unknown, Hyphomonas neptunium jsa2009-06-05-37_slicer8979.jpg: horseshoe vesicle, nested vesicle, stacked membrane? Hyphomonas neptunium jsa2009-06-05-37_slicer8980.jpg: unknown filament, DNA? Hyphomonas neptunium jsa2009-06-05-37_slicer8982.jpg: short tube, Hyphomonas neptunium jsa2009-06-05-37_slicer8984.jpg: unknown, Hyphomonas neptunium

Attached Files

09jun05a_bx467b_067.mrc, Tilt Series (Pixel Size 0.774 nm), 536.9 MB

09jun05a_bx467b_067_part.rec, Reconstruction (Pixel Size 1.548 nm), 419.4 MB

Files

jsa2009-06-05-37_slicer8975.jpg

Files (971.5 MB)

Name Size
536.9 MB (external) Download
419.4 MB (external) Download
md5:63b3d191b84942f5a8f6c97d4e77be1c
91.3 kB Preview Download
md5:8dfbf463c5d226745044361de8078a7f
184.3 kB Preview Download
md5:2fd31eb16d493a8bc29da0016efbbf66
191.7 kB Preview Download
md5:5a7110ecae7b550b53dd3f604296e8d7
187.0 kB Preview Download
md5:2fd3dd88662b72c4046fc797c93317e2
111.7 kB Preview Download
md5:718db3c7cf8d82af281c892bbd73e3f8
1.3 MB Preview Download
md5:934189225d80eb140836a9875502dd15
13.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/t0bfm-9ma73
TILT_SERIES_ID
jsa2009-06-05-37

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-06-05
Created
2009-10-20
Updated
2014-07-30