Published 2014 | Version v1
Dataset Open

V. cholerae delta CheA1,2,3

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Vibrio cholerae strain deleted for CheA1, CheA2, and CheA3

Technical info

Tilt Series Date: 2014-05-05

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Ikuro Kawagishi

Purification / Growth Conditions / Treatment: Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C

Attached Files

VibrioDelta123May0005_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

VibrioDelta123May0005.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

Files

keyimg_aba2014-05-05-5.jpg

Files (4.2 GB)

Name Size
md5:5dbce69102067019c992c515cb4e1632
1.0 MB Preview Download
md5:00a57dc62b32389e5599491fb3a479e3
27.3 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/t1zzp-42057
TILT_SERIES_ID
aba2014-05-05-5

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-05-05
Created
2014-05-05
Updated
2014-09-24