Published 2006
| Version v1
Dataset
Open
Caulobacter crescentus
- Researcher:
- Prabha Dias, D.
Description
wild type CB15N. 4 of 4, not warped. pre-divisional cell with bump instead of stalk; S-layer intact; 2 amorphous blobs: 1 at stalk end, other mid-cell on convex side; short, 2-strand, thick filament at concave side stalk end; large periplasmic 'cap' at non-stalk end where cell touches hole edge
Files
keyimg_pdd2006-08-15-4.jpg
Files
(12.3 MB)
| Name | Size | Actions |
|---|---|---|
|
md5:53b6e753983e7a1ce7db383d17d2fa0e
|
1.0 MB | Preview Download |
|
md5:e6255f11670582c22b0fbb2e7cd50890
|
365.2 kB | Preview Download |
|
md5:6e5f3fc5c50c096fe906a757482c3c8f
|
351.0 kB | Preview Download |
|
md5:d8b3e9d829e5f3c0e46e56edd5b2e867
|
231.3 kB | Preview Download |
|
md5:7749183ec91a3c197ef5badedc0a7d4e
|
277.8 kB | Preview Download |
|
md5:383ddccd7fefdb2322894e7093d06702
|
10.0 MB | Download |
Technical info
Tilt Series Date: 2006-08-15
Data Taken By: Prabha Dias Species / Specimen: Caulobacter crescentus Strain: NA1000 Tilt Series Settings: Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -12.0 μm, magnification: 0x. Acquisition Software: UCSF Tomo Upload Method: rundir Processing Software Used: imod, bsoft Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias Purification / Growth Conditions / Treatment: standard PYE media (Shapiro, Brun, etc. recipe); cultured in 50ml flask; 7 days growth; see DPD notebook Aug 07-14, 2006 Sample Preparation: box 705, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006 SAVE_fil1_xy_2x_01.jpg: file associated to 3D Image #325 SAVE_fil1_yz_2x_01.jpg: file associated to 3D Image #325 pdd2006-08-15-4_slicer10059.jpg: Caulobacter crescentus . granule pdd2006-08-15-4_slicer10060.jpg: Caulobacter crescentus . unknown densityExternal Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-08-15-4/rawdata/
CC06Aug15e150t04.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB Download 06Aug15t04bn_full.rec, Reconstruction (Pixel Size 2.4 nm), 786.4 MB Download SAVE_fil1_xy_2x_01.tif, file associated to 3D Image #325, 5.6 MB Download SAVE_fil1_yz_2x_01.tif, file associated to 3D Image #325, 5.6 MB DownloadAdditional details
- DOI
- 10.22002/t700b-gfz30
- TILT_SERIES_ID
- pdd2006-08-15-4
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2006-08-15
- Created
-
2008-06-18
- Updated
-
2014-08-11