Caltech Tomography Archive
Published 2006 | Version v1
Dataset Open

Caulobacter crescentus

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

wild type CB15N. 4 of 4, not warped. pre-divisional cell with bump instead of stalk; S-layer intact; 2 amorphous blobs: 1 at stalk end, other mid-cell on convex side; short, 2-strand, thick filament at concave side stalk end; large periplasmic 'cap' at non-stalk end where cell touches hole edge

Technical info

Tilt Series Date: 2006-08-15

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias

Purification / Growth Conditions / Treatment: standard PYE media (Shapiro, Brun, etc. recipe); cultured in 50ml flask; 7 days growth; see DPD notebook Aug 07-14, 2006

Sample Preparation: box 705, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006

SAVE_fil1_xy_2x_01.jpg: file associated to 3D Image #325 SAVE_fil1_yz_2x_01.jpg: file associated to 3D Image #325 pdd2006-08-15-4_slicer10059.jpg: Caulobacter crescentus . granule pdd2006-08-15-4_slicer10060.jpg: Caulobacter crescentus . unknown density

Attached Files

CC06Aug15e150t04.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB

06Aug15t04bn_full.rec, Reconstruction (Pixel Size 2.4 nm), 786.4 MB

SAVE_fil1_xy_2x_01.tif, file associated to 3D Image #325, 5.6 MB

SAVE_fil1_yz_2x_01.tif, file associated to 3D Image #325, 5.6 MB

Files

keyimg_pdd2006-08-15-4.jpg

Files (2.3 GB)

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786.4 MB (external) Download
1.5 GB (external) Download
md5:53b6e753983e7a1ce7db383d17d2fa0e
1.0 MB Preview Download
md5:383ddccd7fefdb2322894e7093d06702
10.0 MB Preview Download
md5:d8b3e9d829e5f3c0e46e56edd5b2e867
231.3 kB Preview Download
md5:7749183ec91a3c197ef5badedc0a7d4e
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md5:e6255f11670582c22b0fbb2e7cd50890
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5.6 MB (external) Download
md5:6e5f3fc5c50c096fe906a757482c3c8f
351.0 kB Preview Download
5.6 MB (external) Download

Additional details

Identifiers

DOI
10.22002/t700b-gfz30
TILT_SERIES_ID
pdd2006-08-15-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2006-08-15
Created
2008-06-18
Updated
2014-08-11