gx1-2_sq1_cell4
Description
Technical info
Tilt Series Date: 2018-11-01
Data Taken By: William Nicolas
Species / Specimen: Gluconacetobacter xylinus
Strain: JCM 9730
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 102.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Caltech Titan Krios
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: Overnight culture - centrif 2500rcf, 10min, 30C.Resuspended pellet in 0.5mL at 10:45am
Sample Preparation: Freezing at 4:10pm (5:30 incubation).Quantifoil Cu NH2 Finder R2/2 Thick carbon, Glowdischarged 15mA - 1minManual blotting 5s, glare passed at 3s
Attached Files
gx1-2_sq1_cell4.mrc, Tilt Series (Pixel Size 0.54 nm), 1.7 GB
gx1-2_sq1_cell4_full.rec, Reconstruction (Pixel Size 2.16 nm), 712.7 MB
Files
keyimg_wnj2018-11-01-6.jpg
Additional details
Identifiers
- DOI
- 10.22002/t9eck-zfx36
- TILT_SERIES_ID
- wnj2018-11-01-6
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-11-01
- Created
-
2018-11-02
- Updated
-
2018-12-13