Published 2014 | Version v1
Dataset Open

HeLa

  • 1. ROR icon California Institute of Technology

Description

Synchronized, looking for midbodies

Technical info

Tilt Series Date: 2014-01-05

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: With Doug Mckay and Katherine Ullman

Sample Preparation: HeLa cells+ siRNA NUP 153+ Thymidine 2mM. 14 hour after release

Attached Files

10014.mrc, Tilt Series (Pixel Size 0.6 nm), 861.6 MB

10014_part121_28.rec, Reconstruction (Pixel Size 2.4 nm), 156.6 MB

Files

keyimg_rra2014-01-05-14.jpg

Files (1.0 GB)

Name Size
861.6 MB (external) Download
156.6 MB (external) Download
md5:8bd1b39a5f13455118a671cda6f3c9ee
267.2 kB Preview Download
md5:4e6f270c5d2d678547f179c41523bf6b
27.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/t9sxs-shv33
TILT_SERIES_ID
rra2014-01-05-14

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-01-05
Created
2015-01-06
Updated
2015-01-08