Vibrio cholerae MKW1383
Description
Technical info
Tilt Series Date: 2013-11-06
Data Taken By: Yi-Wei Chang
Species / Specimen: Vibrio cholerae
Strain: 2740-80
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Cells prepared by Lee Rettberg
Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 16 hr at 30 C before freezing
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu R2/2 200 grid
ycw2013-11-06-4_slicer6019.jpg: Flagellar motor? straight ycw2013-11-06-4_slicer6020.jpg: Chemoreceptor ycw2013-11-06-4_slicer6022.jpg: unknown ycw2013-11-06-4_slicer6025.jpg: unknown ring ycw2013-11-06-4_slicer6028.jpg: unknown ring ycw2013-11-06-4_slicer6031.jpg: unknown ring ycw2013-11-06-4_slicer6034.jpg: unknown ring
Attached Files
01-montage_7x70002.mrc, Tilt Series (Pixel Size 0.42 nm), 2.8 GB
01-montage_7x70002_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2013-11-06-4.jpg
Files
(3.5 GB)
| Name | Size | |
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| 2.8 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:f20854af41ba5346f5ccdaea0bdd1107
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md5:776f019c6c6265e3ee91557f1843b181
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md5:4bcd536c82ba6231dbd16f4dd37f7eae
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md5:4bdde8f8b22e08aa370a105286593c6c
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350.4 kB | Preview Download |
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md5:7459a1b1643e59e15026c9e24d93ab76
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md5:dbf83cc4215a4fa6b9335d7d4a8d5573
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250.6 kB | Preview Download |
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md5:47099ac0f89945b68cb2d38545cad7dd
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md5:f491b146cca9c7dbf1577d615f001d40
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md5:bfe750cee1d8bd079123f38e52e26a41
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155.5 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/tfrvs-cg394
- TILT_SERIES_ID
- ycw2013-11-06-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-11-06
- Created
-
2013-11-08
- Updated
-
2014-07-10