Hyphomonas AR46
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-10-08
Data Taken By: Yi-Wei Chang
Species / Specimen: Hyphomonas neptunium
Strain: ATCC 15444
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 75.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSF Tomo
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown overnight in Marine Broth (MB) medium at 30 degrees without antibiotics. OD600=0.6
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
Attached Files
AR46_20121008_10003.mrc, Tilt Series (Pixel Size 0.49 nm), 3.4 GB
AR46_20121008_10003_part122_21.rec, Reconstruction (Pixel Size 1.96 nm), 441.7 MB
Files
keyimg_ycw2012-10-08-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/tjw5q-gjf09
- TILT_SERIES_ID
- ycw2012-10-08-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-10-08
- Created
-
2012-10-08
- Updated
-
2012-11-14