Published 2006 | Version v1
Dataset Open

Caulobacter crescentus

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

wild type CB15N. 6 of 7, some warping during imaging, OK contrast. skinny swarmer cell; no S-layer or obvious filaments; 1 storage granule; central amorphous density along cell length; cell seems twisted about long axis; warping evident at discontinuities in membranes at bottom end

Technical info

Tilt Series Date: 2006-09-26

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias

Purification / Growth Conditions / Treatment: ddi-water ATCC (PYE) media; cultured in 50ml flask; 7 days growth + passage + 5 days growth; see DPD notebook Sep 20 & 25, 2006

Sample Preparation: box 697, grid 2; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 0.5 sec; see DPD notebook Sep 25, 2006

pdd2006-09-26-6_slicer10061.jpg: Caulobacter crescentus . flat vesicle

Attached Files

CC06Sep26t06.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB

CC06Sep26t06_18kx.mrc, Tilt Series (Pixel Size 1.2 nm), 8.4 MB

06Sep26t06bn_full.rec, Reconstruction (Pixel Size 2.4 nm), 796.9 MB

Sep26t06.mov, file associated to 3D Image #360, 303.8 MB

Files

keyimg_pdd2006-09-26-6.jpg

Files (2.6 GB)

Name Size
796.9 MB (external) Download
1.5 GB (external) Download
8.4 MB (external) Download
md5:0eff3c6ba45fa7c7786b9bc536717cab
1.0 MB Preview Download
md5:6f295662613b072d2fa11ff86d81b3b3
9.5 MB Preview Download
md5:2a97ad24ee5b97b291dd0edcd0d378cb
252.1 kB Preview Download
303.8 MB (external) Download

Additional details

Identifiers

DOI
10.22002/tkaxs-t0310
TILT_SERIES_ID
pdd2006-09-26-6

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2006-09-26
Created
2008-06-18
Updated
2014-08-11