Published 2012 | Version v1
Dataset Open

Cryo-sectioned Jurkat

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Jurkats were infected with pNLGFP virus then HP frozen and cryo-sectioned by Mark Ladinsky-Grid4

Technical info

Tilt Series Date: 2012-03-29

Data Taken By: Cora Woodward

Species / Specimen: Jurkat Human T-cell

Strain: Clone E6-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Cryo-sectioning by Mark Ladinsky.

Purification / Growth Conditions / Treatment: infected VSVG-pNL4GFP

Sample Preparation: cryo-sectioned by Mark Ladinsky

Attached Files

12mar29_Jurkatcryosec_tgtsq7_AtlasBT2c_0001_ns_full.rec, Reconstruction (Pixel Size 2.0 nm), 103.2 MB

12mar29_Jurkatcryosec_tgtsq7_AtlasBT2c_0001_ns.st, Tilt Series (Pixel Size 0.5 nm), 635.9 MB

Files

keyimg_cwl2012-03-29-1.jpg

Files (741.7 MB)

Additional details

Identifiers

DOI
10.22002/tmtjj-sfd71
TILT_SERIES_ID
cwl2012-03-29-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-03-29
Created
2015-09-16
Updated
2015-09-16