Published 2014 | Version v1
Dataset Open

Vibrio Stationary Phase

  • 1. ROR icon California Institute of Technology

Description

Vibrio cholerae, deep stationary phase, 37°C, pH 7.5

Technical info

Tilt Series Date: 2014-01-29

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: TRH7000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: MKW1383 grown 28 hours in LB pH 7.5 at 37°C

aba2014-01-29-1_slicer6998.jpg: Unknown flagella with ring aba2014-01-29-1_slicer7001.jpg: Flagella exiting unknown membrane aba2014-01-29-1_slicer7002.jpg: Unknown membrane with ring aba2014-01-29-1_slicer7003.jpg: Unknown filament

Attached Files

VibriostationaryLBmanualblot0001.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibriostationaryLBmanualblot0001_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

aba2014-01-29-1_slicer6998.jpg

Files (4.2 GB)

Name Size
md5:a405face3af9b680ceae513e3d6be9c6
277.8 kB Preview Download
md5:454ac5e9de9263ef1eecf84e34d5c0e2
279.0 kB Preview Download
md5:2fa8bceca6452c0226ae66e7979f79b4
265.3 kB Preview Download
md5:48ae38c88fc88b988f5c47faa02d5cf2
296.8 kB Preview Download
md5:37841332debb5a3f055ac1870e9c1fbb
1.1 MB Preview Download
md5:f3115cf22392dd9d86d9936def698f36
22.9 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/tpkq2-9ka27
TILT_SERIES_ID
aba2014-01-29-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-01-29
Created
2014-01-29
Updated
2014-07-15