H.pylori 26695 delta-cagH

Description

Technical info

Tilt Series Date: 2014-03-02

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori cells from Carrie Shaffer

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

ycw2014-03-02-22_slicer8871.jpg: REZ . Helicobacter pylori ycw2014-03-02-22_slicer8872.jpg: OMV . Helicobacter pylori ycw2014-03-02-22_slicer8873.jpg: OMV . Helicobacter pylori ycw2014-03-02-22_slicer8875.jpg: short tube . Helicobacter pylori ycw2014-03-02-22_slicer8876.jpg: short tube . Helicobacter pylori ycw2014-03-02-22_slicer8878.jpg: flat vesicle ycw2014-03-02-22_slicer8880.jpg: ring . Helicobacter pylori ycw2014-03-02-22_slicer8881.jpg: chemoreceptor . Helicobacter pylori

Attached Files

20140302_YWC090_10022.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB

20140302_YWC090_10022_part131_9.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB