Sulfolobus cells synchronized for division
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2011-01-14
Data Taken By: Megan Dobro
Species / Specimen: Sulfolobus acidocaldarius
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 1.5 eV/Ų, defocus: -15.0 μm, magnification: 18000x.
Acquisition Software: Leginon
Upload Method: rundir
Processing Software Used: Raptor
Collaborators and Roles: grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University
Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine
Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 5 sec, sponge for humidity
Attached Files
11jan14a_Bx8g3_014.mrc, Tilt Series (Pixel Size 1.192 nm), 1.1 GB
11jan14a_Bx8g3_014_part.rec, Reconstruction (Pixel Size 2.5 nm), 943.7 MB
Files
keyimg_mdj2011-01-14-12.jpg
Additional details
Identifiers
- DOI
- 10.22002/v2p4g-tgt60
- TILT_SERIES_ID
- mdj2011-01-14-12
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-01-14
- Created
-
2012-07-09
- Updated
-
2012-07-09