Thermococcus koakarensis
Description
Technical info
Tilt Series Date: 2016-09-20
Data Taken By: Debnath Ghosal
Species / Specimen: Thermococcus kodakarensis
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal
Purification / Growth Conditions / Treatment: Standard Tk medium with starch and sulfur
Sample Preparation: 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.
Attached Files
atlas10005.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
atlas10005_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB
Files
keyimg_dga2016-09-20-6.jpg
Additional details
Identifiers
- DOI
- 10.22002/v7tjx-cg251
- TILT_SERIES_ID
- dga2016-09-20-6
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-09-20
- Created
-
2016-09-21
- Updated
-
2016-09-23