Published 2012 | Version v1
Dataset Open

E. coli (FB76)

Creators

  • 1. ROR icon California Institute of Technology

Description

Contains MreB-mCherry sandwich fusion as sole source of MreB from WT promoter.

Technical info

Tilt Series Date: 2012-05-07

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 23000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Gift from Piet de Boer

Purification / Growth Conditions / Treatment: LB, 30 Degrees Celsius, OD600 ~ 0.6

mst2012-05-07-13_slicer9637.jpg: Escherichia coli . peptidoglycan

Attached Files

20120507_FB76_M10.mrc, Tilt Series (Pixel Size 0.95 nm), 1.1 GB

20120507_FB76_M10_part130_29.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB

Files

keyimg_mst2012-05-07-13.jpg

Files (1.9 GB)

Name Size
1.1 GB (external) Download
825.8 MB (external) Download
md5:55244bdb0f0d2641c09a21b2ace0c4a6
1.2 MB Preview Download
md5:7b62338d2b093fd6dfe3b29ab5368df5
24.2 MB Preview Download
md5:f21e787ca7e7041beadc6cb2f145b719
472.1 kB Preview Download

Additional details

Identifiers

DOI
10.22002/v8qpw-kn043
TILT_SERIES_ID
mst2012-05-07-13

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-05-07
Created
2012-05-08
Updated
2014-08-07