Caltech Tomography Archive
Published 2013 | Version v1
Dataset Open

Helicobacter pylori 26695

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Cells are sick. Don't have flagella. Only see partial motors.

Technical info

Tilt Series Date: 2013-10-09

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Cell prepared by Lee Rettberg.

Purification / Growth Conditions / Treatment: Cells grown in Brucella broth (Fluka) + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2, OD600=0.4

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200

Attached Files

20131009_Hpylori_26695_BB_10026.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB

20131009_Hpylori_26695_BB_10026_part131_14.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB

Files

keyimg_ycw2013-10-09-26.jpg

Files (4.5 GB)

Name Size
3.7 GB (external) Download
712.7 MB (external) Download
md5:df3d293991f553eb9be015aace45049b
1.1 MB Preview Download
md5:00e2ccf689a155b16dbe8f516ad1a273
23.8 MB Preview Download

Additional details

Identifiers

DOI
10.22002/vdwvt-22951
TILT_SERIES_ID
ycw2013-10-09-26

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-10-09
Created
2013-10-10
Updated
2013-10-17