Myxococcus xanthus DK1622 wt
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-03-27
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Acquisition Software: FEIbatchtomo
Upload Method: pipeline
Processing Software Used: FEIBatchTomo
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200
Attached Files
DK1622_UCLA_20130327_17.mrc, Tilt Series (Pixel Size 1.352 nm), 1.1 GB
DK1622_UCLA_20130327_17_full.rec, Reconstruction (Pixel Size 2.704 nm), 838.9 MB
Files
keyimg_ycw2013-03-27-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/vezfg-6em44
- TILT_SERIES_ID
- ycw2013-03-27-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-03-27
- Created
-
2013-03-29
- Updated
-
2013-04-25