Skinny E. coli with GFP-MreB

Description

Technical info

Tilt Series Date: 2010-03-05

Data Taken By: Zhuo Li

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -12.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR and Peach

Collaborators and Roles: Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.

Purification / Growth Conditions / Treatment: L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)

Sample Preparation: R2/2 CF or Quantifoil, 10 nm gold, 100% humidity

Attached Files

10mar05a_corr_gm_J_005.mrc, Tilt Series (Pixel Size 1.192 nm), 721.4 MB