E.cloacae-typeV-CdiA+
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2014-03-03
Data Taken By: Poorna Subramanian
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Drs. Davod Low and Chris Hayes, UC Santa Barbara
Purification / Growth Conditions / Treatment: Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning
Sample Preparation: Blot time 3s, Blot off-set -4, 3 ul culture
psa2014-03-03-16_slicer3765.jpg: type V
Attached Files
atlas10016.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
atlas10016_full.rec, Reconstruction (Pixel Size 1.92 nm), 801.8 MB
Files
keyimg_psa2014-03-03-16.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.4 GB | (external) Download | |
| 801.8 MB | (external) Download | |
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md5:2f41e58b6d90ff405b62ef10694e420a
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1.1 MB | Preview Download |
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md5:07ba4b0d7ac70c7c7d02185f8bfd35fa
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25.9 MB | Preview Download |
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md5:1ece7f1694886ca2f85e011ff20d4da9
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719.2 kB | Preview Download |
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md5:e35782a6ee6d6bdf56d2057946bbaebf
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612.6 kB | Preview Download |
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md5:6bf855e559f0d4ac5eeaca2cb9e72f29
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713.2 kB | Preview Download |
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md5:c7d5b6f913b83b204761be9acb4edc64
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599.9 kB | Preview Download |
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md5:4a5e7246e844c9ca9c1ce50545f5c958
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632.4 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/vgfr2-wk571
- TILT_SERIES_ID
- psa2014-03-03-16
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-03
- Created
-
2014-03-04
- Updated
-
2017-01-12