Published 2017 | Version v1
Dataset Open

E.coli minicell/Pul T2SS

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

grid box YWC252strain PAP5359 (minCDE knockout)Cells were grown on plate before freezing

Technical info

Tilt Series Date: 2017-05-15

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain provided by Ingrid Guilvout in Olivera Francetic lab

Attached Files

20170515_YWC252b_10065.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

20170515_YWC252b_10065_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB

Files

keyimg_ycw2017-05-15-60.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:2a99aede071ba459e90b3916fd3eb1ff
1.0 MB Preview Download
md5:b6d4398b38467522551aff095aaaabdc
17.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/vgp1s-87841
TILT_SERIES_ID
ycw2017-05-15-60

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-05-15
Created
2017-05-17
Updated
2017-05-30