Myxococcus xanthus delta-tsaP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-09-15
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 3 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot (MK vi) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 3, blot time 3 sec. Wait time 10 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20140915_YWC115_30036.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
20140915_YWC115_30036_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB
Files
keyimg_ycw2014-09-15-43.jpg
Additional details
Identifiers
- DOI
- 10.22002/vgq0f-rj460
- TILT_SERIES_ID
- ycw2014-09-15-43
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-09-15
- Created
-
2014-09-16
- Updated
-
2014-09-27