Xanthomonas WT
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2019-12-06
Data Taken By: Debnath Ghosal
Species / Specimen: Xanthomonas citri
Strain: pv citri 306
Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 19500x.
Acquisition Software: SerialEM
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Carvalho and Farah lab sent the strain. Debnath Ghosal collected data
Purification / Growth Conditions / Treatment: Cells were grown in 2xTY with apt antibiotics, freeze cells next day after about 14 hour when they are in stationary phase.
Sample Preparation: Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 5 sec. ** Use 7 sec for next sessions.
Attached Files
Xac_No1_TS39.mrc, Tilt Series (Pixel Size 0.353 nm), 1.7 GB
Xac_No1_TS39_full.rec, Reconstruction (Pixel Size 1.412 nm), 737.3 MB
Files
keyimg_dga2019-12-06-29.jpg
Additional details
Identifiers
- DOI
- 10.22002/vheya-baq71
- TILT_SERIES_ID
- dga2019-12-06-29
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-12-06
- Created
-
2020-06-10
- Updated
-
2021-06-28