Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

Legionella KS79 (WT), 42 C_On

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology

Description

Legionella KS79 (WT),42 C_On (HS001 A)

Files

keyimg_dga2019-05-28-190.jpg
Files (16.0 MB)
Name Size
keymov_dga2019-05-28-190.mp4
md5:3364278001f79d3fe57dde555e689c56
15.0 MB Download
keyimg_dga2019-05-28-190.jpg
md5:1d0b633d0480fc79bca27ac537fbf1b2
1.1 MB Preview Download

Technical info

Tilt Series Date: 2019-05-28

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 42000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Howard Shuman (University of Chicago). Data collected by Debnath Ghosal and Yuxi Liu.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-05-28-190/rawdata/

HS001A-area2-tomo0015.mrc, Tilt Series (Pixel Size 0.26 nm), 996.7 MB Download

HS001A-area2-tomo0015_full.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB Download

Additional details

Identifiers Funding Dates
Created:
June 4, 2023
Modified:
August 26, 2025